The smear microscopy is the most rapid method for detection of acid-fast bacilli (AFB) in clinical specimens. Smears detect viable and non-viable organisms; with a threshold of 10,000 organisms /ml of sputum, .This Centre receives 80 to 120 pulmonary and Extra-Pulmonary specimens per day. The specimens were digested and decontaminated by petroff 's (using 4% NAOH) method. The results of smear microscopy mailed or faxed to other hospital , correctional facilities, and intermediate laboratories of Iran immediately .
Smear : indirect after decontamination by Petroff method Staining : Ziehl-nelson staining
